ABSTRACT
Context: the Tehran Lipid and Glucose Study [TLGS] is a community-based study to reveal the frequency of non-communicable diseases [NCDs] in Tehran's population. This research consists of two main parts, a cross-sectional study on the prevalence of cardiovascular risk factors and a 20-year-ongoing prospective cohort study, which was initiated in 1999 in several phases with an approximate duration of 3.6 years, and is still ongoing. The aim of the present study is review the 20 year biochemical findings of the TLGS related to the NCDs in a large sample
Methods: all articles on biochemical assessments derived from the TLGS from the earliest publications [2002] until 30 January 2018 were reviewed for their findings on different risk factors of NCDs
Results: according to the TLGS findings high sensitivity C-reactive protein [hs-CRP], interleukin-6 [IL-6], homocysteine [Hcy], age, smoking, hypertension, and obesity were the most important risk factors of cardiovascular diseases [CVD]. It was illustrated that in subjects with abdominal obesity, the hs-CRP and IL-6 serum levels were higher than in normal subjects. The most appropriate prognostic indexes and associations were for hs-CRP, IL-6, and Hcy with abdominal obesity, waist circumference, WHtR, and wrist circumference, respectively. Previous studies have demonstrated a direct relationship between obesity and serum levels of inflammatory factors
Conclusions: according to the results of TLGS, serum levels of biochemical risk factors such as hs-CRP, IL-6, and Hcy could be beneficial in early diagnosis and effective treatment of cardiovascular, obesity and other metabolic diseases
ABSTRACT
Background: Medullary thyroid carcinoma [MTC] occurs in both sporadic [75%] and hereditary [25%] forms. The missense mutations of the RE arranged during Transfection [RET] proto-oncogene in MTC development have been well demonstrated. The aim of this study was to investigate frequency of G691S/S904S haplotype in MTC patients and their relatives
Methods: In this research 293 participants were studied, including 181 patients [102 female, 79 male] and 112 their relatives [58 female, 54 male]. Genomic DNA was extracted from peripheral blood leucocytes using the standard Salting Out/Proteinase K method. Nucleotide change detection was performed using PCR and direct DNA sequencing methods
Results: According to DNA sequencing results, 159 individuals [104 patients, 55 relatives] had both G691S [rs1799939] missense mutation in exon11 and S904S [rs1800863] synonymous mutation in exon 15 of RET proto-oncogene. The allele frequency of G691S/S904S haplotype was 21.15% in patients and 10.75% in their relatives
Conclusion: The obtained data showed the frequency of G691S/S904S RET gene haplotype among Iranian MTC patients and their relatives. The G691S and S904S nucleotide changes were in complete linkage disequilibrium, so the results were grouped together and referred to as G691S/S904S haplotype. Further analysis is need to demonstrate the association between this haplotype and MTC development